This technology meets the goal of green and sustainable criteria by developing innovative indigo dye producing bacteria, using sustainable materials, and utilizing ambient production conditions to produce the biosynthetic dyes. This technology overcomes the dilemma of non-scalable nature of plant-derived dyes; at the same time, it completely eliminates the concern stemmed from the hazardous chemical intermediates from the chemical dye synthesis; thus becoming the transformative core technology for the textile industry. In collaboration with JINTEX Co., Ltd., we have developed the dispersion formulation of the biosynthetic indigo dyes that is comparable with existing chemical dye formulation. The dye has been used to dye 1,200 yards of denim, the dyeing performance meet meets the manufacturer’s specifications. This technology is recognized as Silver Winners of the 2021 Edison Awards.