A high-throughput, highly sensitive, precise, and cost-effective microwell protein microarray chip and reader is developed. This devise can be applied to the multi-biomarkers detection of neurodegenerative diseases, directly quantifying the levels of blood protein molecules. This has the potential to improve the diagnosis and treatment of neurodegenerative diseases, enhance the quality of life for patients, and reduce long-term care costs.eating a three-in-one technology and system that contributes significantly to Taiwan's technological integration and innovation.