Culture media is a key raw material in the biotechnology field. However, China lacks key research and development capabilities and production experience for culture media and has long relied on foreign imports. In stem cell medicine, the world lacks the technology to effectively produce mesenchymal stem cells in vitro. We use our self-developed high-efficiency serum-free culture medium to establish stem cell mass production process technology to fill the industry gap, and the cell production capacity is better than existing international production technology.